Dear Visitor,

On this website ROZING.COM Consulting, I have collected documents that are dealing with basics and important aspects of pressure and electrical field driven liquid phase separations.

The documents that I have prepared, have been a lot of work. Allthough much of their contents can be found in text books and publications, it has been a large task to arrange and prepare the content and make them available for your study and information. Therefore access to the menus one these pages e.g. to PDF-copies of my talks, tutorials and reports are only available after registration. To register, just send me a mail through the contact form.

In the content of this blog following this article, I will announce important news regarding ROZING.COM Consulting and will refer to all new content on the site. So check this blog for new information. In addition, I will mail the registered visitors occasionally.

Just another hint. Some of the articles, like this one, have tags. Clicking one of this tags will show you all pages relevant to the text on the tag. E.g. try the tag "Tutorial" or "Reprints". Note: if you are not logged in, only this page is returned. Make sure to have an account on this site to access the Tutorials or my Reprints.

So, if you find something interesting in the blog and/or want more information or just want to stay informed about new content added to this website, please contact me through the contact form.

   Gerard Rozing

Two awards (600 Euro ea) for HPLC2017 - Prague are made available by ROZING.COM Consulting for PhD or post-doctoral students with an accepted oral or poster contribution for participation in international symposia dealing with fundamentals of (U)HPLC, column technology, Capillary Electrophoresis or new concepts in liquid phase separation science at the conference. The grant furnishes funding for travel and/or for the registration fee. For information about the symposium and the actual offering see for more details from the symposium organizers.


General requirements for application for a travel grant are:

  • Proof of registration to the conference and acceptance of your abstract for a poster or oral presentation
  • Proof of studentship/post-doc status
  • A letter of recommendation from your supervisor.
  • A letter with your reasons for requesting the grant.
Please use the contact form on this web site to obtain information on the actual submission of your application. Deadline for submission of the application is May 24.
The grants are sponsored by Dr. Gerard Rozing, 33 years with Hewlett-Packard and Agilent Technologies Germany and since 2012 an independent consultant for Liquid Chromatography and Capillary Electrophoresis. With these grants Dr. Rozing intends to return the enjoyment, enthusiasm and motivation in (U)HPLC and CE technology he has encountered at past HPLC conferences in over 30 times attendance.

At the 31st International Symposium on Chromatography, I had the priviledge to arrange the reviews for the Genzo Shimadzu Best Poster Award. Together with an international board of senior peers participating in the Symposium the posters were reviewed by the following criteria:

  • Novelty and originality of the work, creativity and potential for innovation
  • Scope of work, technical quality of experimental design and execution of experiments
  • Readability of the presentation and author’s explanations (during the poster session!)

Approx. 350 posters have been submitted for presentation at ISC2016. About 150 authors have opted to participate in the best poster award competition. After  first round poster evaluation, by 16 reviewers working in pairs, 14 contributions were inivited for final round evaluation. This evaluation took place in a plenary session on Thursday, September 1 from 8:30 – 10:00 hrs, during which the nominated authors gave a flash presentation of her/his work in max. 3 to 4 slides. Fifteen reviewers in the room ranked the poster presentations and presenters which resulted in an overall rank.

It is my pleasure to announce a new monograph on CE-MS

CE-MS Book


Detection in Capillary Electrophoresis – an Introduction
Gerhardus de Jong 

Electrospray Ionization Interface Development for Capillary Electrophoresis-Mass Spectrometry
Jessica M. Risley, Caitlyn A.G. De Jong, David D.Y. ChenD

Sheath Liquids in CE-MS: Role, Parameters and Optimization
Christian W. Klampfl and Markus Himmelsbach

Recent Developments of Microchip Capillary Electrophoresis Coupled with Mass Spectrometry
Gerard Rozing

On-line Electrophoretic, Electrochromatographic, and Chromatographic Sample Concentration in CE-MS
Joselito P. Quirino

CE-MS in Drug analysis and Bioanalysis
Julie Schappler, Víctor González-Ruiz, Serge Rudaz

Intact Protein Analysis by Capillary Electrophoresis – Mass Spectrometry
Rob Haselberg and Govert W. Somsen

The 42nd International Symposium on High Performance Liquid Phase Separations and Related Techniques took place in Geneva, June 21-25 2015. As at all recent HPLC symposia there was a Best Poster Award competition which was sponsored by Agilent Technologies. Also like in previous years, I had the privilege to organize the poster reviews and selection of awardees.

Of around 600 submitted posters approx. 300 authors have opted to participate in the Best Poster Award competition.  This number had to be reduced to the 10 best, who were rewarded with the Best Poster Award certificate and a cash prize of 500 Euro. 

Due to space limitations, all posters presented were divided into two groups, PSA- and PSB-series. Posters in the PSA-series were on display Monday and Tuesday; posters in the PSB-series were on display Wednesday and Thursday. There were three poster sessions every day (but for the last day Thursday). Authors were asked to be at their poster at designated sessions.

It is my pleasure to announce my involvement with Advanced Electrophoresis Solutions as a scientific advisor. 

Focused on providing cutting edge instrumentation, ampholytes and supplies, as well as superior customer support, AES recently has successfully commercialized uniques technologies and is now able to provide their customers with  CIEF (Capillary isoelectric focusing) related products such as proprietary WCID (Whole Column Imaging Detection) based high performance CIEF instruments, WCID imaging cartridges (patent pending), reference materials, electrolytes and polymer solutions. AES products are the result of the extensive dedication of scientists and engineers continuously working in the fields of liquid phase separation science electrophoresis, chromatography and analytical analysis.

September 30th I participated in the first Sciex Separations CESI-MS Users Meeting. The meeting was hosted by  Ass. Prof. Yannis Francois at Strasbourg University.  The meeting was organized by Drs. Stephen Lock and Jim Thorn of Sciex Separations.

CESI marks a novel combination of capillary electrophoresis (CE) and electrospray ionization (ESI) technologies to create an integrated workflow solution. The CE part consists of the former Beckman-Coulter PA800 CE System for Biologics Characterization and a choice of AB Sciex MS systems like the TripleTOF® 5600+ mass spectrometry system. The interface is based on the porous tip approach described by Moini (Anal. Chem. 2007, 79, 4241 - 4246) which has been extensively beta-tested by Beckman-Coulter (see e.g. Anal. Chem. 2010, 82, 9476–9483). With the formation of Sciex Separations, by combining the HPLC separation business of Eksigent with the CE separation business of Beckman Coulter, seamless complete offering for LC-MS and CE-MS is now commercially available by combining the best from Sciex MS portfolio e.g. AB Sciex TripleTOF® 5600+ mass spectrometry system.

More information can be found at the

Sciex Separations website.

At the 30th International Symposium  on Chromatography which took place September 14-18 in Salzburg, Austria I had the priviledge to chair a group of peers who volunteered to help select the best posters presented. Close to 600 posters were presented at the symposium of which 286 have opted to compete for the award. The Best Poster Award competition was sponsored by Genzo Shimadzu and consisted of eight 500€ grants. So it was my task to reduce the group of 286 to the eight best contributions. Banner ISC2014

I  was invited to contribute to the above distinguished seminar, arranged by Thermo Fisher Scientific. The event took place at the Thermo Fisher Scientific site in Germering close to Munich, September 4 and 5. The seminar was arranged and moderated by Dr. Frank Steiner and Dr. Remco Swart. Several well known scientists were present like Prof. David McCalley, Univ. South West England, Prof. Wolfgang Lindner, Vienna University, Prof. Christian Huber from Salzburg University, Prof. Gert Desmet, Free University of Brussels, Prof. Gérard Hopfgartner from Geneva University, Prof. Peter Schoenmakers, University of Amsterdam. Presentations were delivered by David McCalley,  Christian Huber, Dr. Stephan Lamotte, Dr Thorsten Teutenberg, Univ. of Duisburg, Dr. Bas Eeltink, Free University of Brussels, Dr. Chris Pohl of Thermo Scientific and myself. A PDF-copy of my talk can be found in the menu item "My Orals".

Dear Visitor,

I have prepared a comprehensive blog on CE-MS interfacing methods. Actually the information provided is distributed over three separate blogs viz. "CE-MS, the first steps", "The triple tube IF" and "Sheathless CE-MS IF". The first blog describes how CE-MS interfacing was developed until the HP/Agilent triple tube CE-MS interface was introduced on the market. The second blog has a few articles about the triple tube interface while the third blog describes new development and gives my assessment of the current status in CE-MS interfacing.


The 41th Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC2014, took place in the Hilton Riverside Hotel, New Orleansand from May 11-15. The symposium was attended by approx. 770 delegates. About 60-70 of these were registered as exhibitors. Accounting for invited delegates, there were about 600 paying delegates. More than 200 orals and over 450 posters were presented. Ca. 45 manufacturers exhibited besides 20 media partner. These numbers are significantly lower than at HPLC2013 in Amsterdam.

The 30th International Symposium on MicroScale Bioseparations (MSB2014) took place in Pécs, Hungary, April 27 - May 1. The meeting was organized by Prof. Ferenc Kilár, who was the symposium chairman together with Profs. Attila Fellinger and András Guttman as co-chairmen under the auspices of the University of Pécs. The MSB symposium traditionally focuses on fundamental aspects of separations on the microscale or down to nanoscale. The scientific areas of the symposium cover the application of electrically driven, microscale separations for (bio)analytical  measurements  in  life  science  research,  (bio)pharmaceuticals  development,  forensics, toxicology,  doping  analyses,  food  safety  and  authenticity  and  keeping  our  environment  clean  and sustainable. See the symposium website for more information.